The complete sequence of the group-specific antigen, VP7, of African horsesickness disease virus serotype 4 reveals a close relationship to bluetongue virus.

Structural insight into African horsesickness virus infection.

African horsesickness (AHS) is a devastating disease of horses. The disease is caused by the double-stranded RNA-containing African horsesickness virus (AHSV). Using electron cryomicroscopy and three-dimensional image reconstruction, we determined the architecture of an AHSV serotype 4 (AHSV-4) reference strain. The structure revealed triple-layered AHS virions enclosing the segmented genome an...

The complete sequence of African horsesickness virus serotype 4 (vaccine strain) RNA segment 5 and its predicted polypeptide compared with NS1 of bluetongue virus.

The complete sequence of RNA segment 5 of the African horsesickness virus serotype 4 (AHSV-4) vaccine strain was determined from cDNA clones inserted into pBR322. The RNA is 1751 bp long (M(r) 1.12 x 10(6)) and contains an open reading frame encoding a protein of 548 amino acids (M(r) 63,122) with a net charge of +0.5 at neutral pH. A comparison of the sequence of AHSV-4 segment 5 with that of ...

The complete nucleotide sequence of genome segment 7 of bluetongue virus, serotype 1 from South Africa.

The complete sequence of genome segment 7, of bluetongue was 98.3% identity and 98.9% similarity (including conservative virus, serotype 1 South Africa (BTV1SA), which encodes the changes). serogroup-specific antigen, VP7 (1), was determined from a full length cDNA clone synthesized using terminal oligonucleotides REFERENCES as primers (2). Genome segment 7 from BTV1SA is the same length as the...

Production and properties of monoclonal antibodies against African horsesickness virus, serotype 3.

Four polyethylene glycol-mediated cell fusions yielded a total of 23 monoclonal antibodies (McAbs) specific for African horsesickness virus (AHSV). Two recognised the major core structural polypeptide, VP7, while one each was specific for the outer capsid proteins, VP2 and VP5. The remainder co-precipitated both VP2 and VP7. An inhibition ELISA and radio-immunoprecipitation revealed two types o...

The study of African horsesickness virus by the agar double-diffusion precipitation test. Characterization of the precipitation antigen